Part:BBa_K2922000:Design

Beta-D-glucosidase fused at N-terminal with YebF secretion protein

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1591
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Overlap extension PCR from yebf gene and beta-glucosidase gene.

Source

YebF comes from Escherichia coli (Strain K12) and bgl1A is from Saccharophagus degradans 2-40.